ISO INTERNATIONAL STANDARD 19020 First edition 2017-06 Microbiology of the food chain - Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs Microbiologie de la chaine alimentaire - Méthode horizontale de détection des enterotoxines staphylococciques par test immuno- enzymatique dans les aliments Reference number IS0 19020:2017(E) os1 International Organization for Standardization @ IS0 2017 =ZHEJIANG INST OF STANDARDIZATION C1 5956617 vided by IHS under I IS0 19020:2017(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org PrganizationforStandardization icensee-ZHEJIANG INST OF STANDARDIZRoISQ s1.7 - All rights reserved Not for Resale, 2017/8/21 01:39:05 etworking permitted without license from IHS IS0 19020:2017(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Reagents. .2 6 Apparatus. ..2 7 Sampling. ..3 8 Procedure .3 8.1 Preparation of test portion .3 8.2 Storage of the test sample 3 8.3 Extraction. .4 8.4 Concentration of the extract (mandatory for milk and dairy products) .5 8.5 Recovery of the concentrated extract .5 8.6 Storage and steps before detection .6 8.7 Detection. .6 8.8 Performance criteria 9 Quality control. ..6 10 Expression of results. ..7 11 Confirmation .1 12 Performance characteristics of the method > 13 Test report ..9 Annex A (informative) Results of interlaboratory studies: 2013 ..10 Annex B (informative) Results of interlaboratory studies: 2014 ..15 Annex C (informative) Note on interferences ..21 Bibliography .22 iii ee=ZHEJIANG INSTOFSTANDARDIZATION C15956617 ithout license from IHS