EN 12469 EUROPEANSTANDARD NORMEEUROPEENNE EUROPAISCHENORM May2000 ICS 07.080; 07.100.01 English version Biotechnology - Performance criteria for microbiological safety cabinets Biotechnologie : Criteres de performance pour les postes Biotechnik - Leistungskriterien fur mikrobiologische de securite microbiologique Sicherheitswerkbanke This European Standard was approved by CEN an 3 January 2000. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-datelists and bibliographical references concerning suchanational standards may be obtained.on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation versions. CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, cen EUROPEAN COMMITTEE FOR STANDARDIZATION COMITE EUROPEEN DE NORMALISATION EUROPAISCHESKOMITEEFURNORMUNG Central Secretariat: rue de Stassart, 36 B-1050 Brussels 2000CEN Ail rights of expioitation in any form and by any means reserved Ref.No.EN12469:2000 E worldwide for CEN national Members. Page 2 EN12469:2000 Contents Page Foreword Introductio Scope Normativereferences Definition Hazards Performance classes. Safety requirements Marking and packaging... 13 Documentation 14 Annex A (informative) Guidance on materials, design and manufacture... 15 Annex B (normative) Test method for leakage of carcass for class I and II MsCs.... 18 aperture Annex D (informative) Aerosol challenge test method for installed HEPA filter systemleakagedetection Annex E (normative)Test method for product protection for class Il MsCs Annex F (normative) Test method for cross contamination protection for class Il MSCs... Annex G(informative)Method of measurement ofvolumetric airflow rate. 36 Annex H (informative) Design of MsCs and airflow velocities.in MsCs.. ..38 Annex J(informative)Recommendationsfor decontamination,cleaning and fumigationofMsCsandfilters.. .40 AnnexK (informative)Recommendations for routinemaintenance of MsCs 42 Bibliography... Page 3 EN12469:2000 Foreword "Biotechnology", the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 20oo, and conflicting national standards shall be withdrawn at the latest by November 2000. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom. Page 4 EN12469:2000 Introduction Microbioiogical safety cabinets are intended to reduce the risk to the operator when handling hazardous or potentially hazardous microorganisms. They do not necessarily protect the operator from all hazards involved. Some types of safety cabinet can also protect the materials being handled in them from environmental contamination. 1 Scope This European Standard specifies basic requirements for microbiological safety cabinets (MsCs) with respect to safety and hygiene. This European Standard sets the minimum performance criteria for safety cabinets for work with microorganisms and specifies test procedures for microbiological safety cabinets with respect to protection of the worker and the environment, product protection and cross contamination. Mechanical, electrical, chemical or radioactive safety precautions are not Bibliography [1], [2] and [3]. This European Standard does not cover safety precautions for aspects not associated with the use of microorganisms such as those cover