论文标题
GABA和谷氨酸的体内磁共振成像
In-vivo Magnetic Resonance Imaging of GABA and Glutamate
论文作者
论文摘要
化学交换饱和转移（CEST）磁共振成像（MRI）是一种分子成像方法，能够映射具有相对较高空间分辨率的脑代谢产物。特异性是此类实验的主要目标。然而，CEST被不同分子物种之间的光谱重叠所混淆。在这里，我们使用称为重叠的CEST（ORCEST）的通用框架来克服这一主要限制 - 一种频谱编辑的实验恢复了特异性。首先，我们提出证据表明，针对中枢神经系统的原发性兴奋性神经递质谷氨酸（Glucest）的CEST实验受到γ-氨基丁酸（GABA）的显着污染 - CNS中的一级抑制性神经递质。然后，我们利用新型的orcest方法来分离谷氨酸，并首次将GABA信号分开，从而提供所需的特异性。体内孔的实验解决了大鼠脑的主要神经递质，并揭示了在口渴相关区域缺水后谷氨酸和GABA水平的变化。 Orcest的特征bode很好地用于神经科学和生物医学中的许多应用。
Chemical Exchange Saturation Transfer (CEST) Magnetic Resonance Imaging (MRI) is a molecular imaging methodology capable of mapping brain metabolites with relatively high spatial resolution. Specificity is the main goal of such experiments; yet CEST is confounded by spectral overlap between different molecular species. Here, we overcome this major limitation using a general framework termed overlap-resolved CEST (orCEST) - a kind of spectrally-edited experiment restoring specificity. First, we present evidence revealing that CEST experiments targeting the central nervous system's primary excitatory neurotransmitter, Glutamate (GluCEST) - is significantly contaminated by gamma-aminobutyric acid (GABA) - the primary inhibitory neurotransmitter in the CNS. Then, we harness the novel orCEST methodology to separate Glutamate and - for the first time - GABA signals, thus delivering the desired specificity. In-vivo orCEST experiments resolved the rat brain's primary neurotransmitters and revealed changes in Glutamate and GABA levels upon water deprivation in thirst-related areas. orCEST's features bode well for many applications in neuroscience and biomedicine.
