论文标题

内部微观镜和纳奎斯特极限之外

Endo-microscopy beyond the Abbe and Nyquist limits

论文作者

Amitonova, Lyubov V., de Boer, Johannes F.

论文摘要

几个世纪以来,远场光学显微镜一直是许多科学学科(包括物理,化学和生物医学研究)的关键工具。但是,远场成像具有许多局限性:空间分辨率受光的衍射控制,成像速度遵循奈奎斯特香农采样定理。超分辨率技术的最新发展推动了空间分辨率的局限性。但是,这些方法通常需要复杂的设置,较长的获取时间,并且仍然不适用于深度组织生物成像。在这里,我们通过超出ABBE限制的空间分辨率的超薄纤维探针报告成像,并在简单且紧凑的设置中同时使用超出Nyquist极限的时间分辨率。我们在多模纤维,稀疏性约束和压缩传感重建中使用模式耦合的随机性质。超分辨率内部显微镜检查的新方法不使用荧光标签的任何特定特性,例如分子荧光态的消耗或随机激活,因此可以用于无标记成像。我们证明了空间分辨率比衍射极限和成像速度高20倍的空间分辨率比Nyquist极限快20倍。所提出的方法可以显着扩大纳米镜检查用于生物成像的领域。

For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial resolution is controlled by the diffraction of light and the imaging speed follows Nyquist-Shannon sampling theorem. The recent development of super-resolution techniques has pushed the limits of spatial resolution. However, these methods typically require complicated setups, long acquisition time and are still not applicable for deep-tissue bioimaging. Here we report imaging through an ultra-thin fiber probe with a spatial resolution beyond the Abbe limit and a temporal resolution beyond the Nyquist limit simultaneously in a simple and compact setup. We use the random nature of mode coupling in a multimode fiber, the sparsity constraint and compressive sensing reconstruction. The new approach of super-resolution endo-microscopy does not use any specific properties of the fluorescent label such as depletion or stochastic activation of the molecular fluorescent state and therefore could be used for label-free imaging. We demonstrate a spatial resolution more than 2 times better than the diffraction limit and an imaging speed 20 times faster than the Nyquist limit. The proposed approach could significantly expand the realm of the application of nanoscopy for bioimaging.

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