论文标题
转录分析揭示了内皮细胞IPS衍生的祖细胞（PEC）与成人循环EPC的基本差异
Transcriptional profiling reveals fundamental differences in iPS-derived progenitors of endothelial cells (PECs) versus adult circulating EPCs
论文作者
论文摘要
有许多不同的干细胞来源有可能用作血管退行性疾病的治疗剂。一方面，有所谓的内皮祖细胞（EPC）通常源自成人血液。他们携带标记CD34，但EPC的真实性质和定义仍然存在争议。另一方面，还有内皮细胞（PEC）的胚胎前体，它们也表达CD34，并且可以在体外与胚胎干细胞（ESC）或诱导的多能干细胞（IPSC）区分开。在这项研究中，它的目的是通过完整的基因组转录分析（RNASEQ）比较这两个不同的CD34阳性细胞群。为此，我们首先优化了PEC分化方案，发现血管内皮生长因子（VEGF）对于从中胚层前体向PEC的过渡至关重要。此外，RNASEQ数据的主要成分分析（PCA）表明，血液来源的EPC与IPS衍生的PEC群群群群群远，这些PEC说明了这些人群的根本不同。这些数据将有助于更好地定义这些细胞群体并促进该领域再生方法的翻译以及提供潜在的新型诊断工具。
There are a number of different stem cell sources that have the potential to be used as therapeutics in vascular degenerative diseases. On the one hand, there are so called endothelial progenitor cells (EPCs), which are typically derived from adult blood. They carry the marker CD34, but the true nature and definition of EPCs is still controversial. On the other hand, there are embryonic precursors of endothelial cells (PECs), which also express CD34, and can be differentiated from embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in vitro. In this study, it was aimed to compare these two different CD34 positive cell populations by full genome transcriptional profiling (RNAseq). To this end, we firstly optimised a PEC differentiation protocol and found that vascular endothelial growth factor (VEGF) is critical for the transition of cells from mesodermal precursors to PECs. Additionally, principal component analysis (PCA) of RNAseq data showed that blood-derived EPCs clustered far from iPS-derived PECs which illustrates these populations are fundamentally different. This data will be useful to better define these cell populations and facilitating the translation of regenerative approaches in this field as well as providing potentially novel diagnostic tools.
