论文标题

使用共焦和相对比显微镜的巨型单层囊泡的波动光谱

Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

论文作者

Faizi, Hammad A., Reeves, Cody J., Georgiev, Vasil N., Vlahovska, Petia M., Dimova, Rumiana

论文摘要

一种广泛使用的方法来测量双层膜的弯曲刚度是波动光谱,该方法分析了用相位对比度或共焦显微镜记录的巨型Unilamelar囊泡的热驱动的膜起伏。在这里,我们使用这两种技术分析了相同囊泡的波动,并获得了弯曲模量的一致值。我们讨论可能导致差异的因素。

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

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