论文标题
用于单个活细胞中活性氧和氮种的细胞内测量的纳米电极
Nanoelectrodes for intracellular measurements of reactive oxygen and nitrogen species in single living cells
论文作者
论文摘要
活性氧和氮种（ROS和RN）在各种生理过程（例如吞噬作用）和病理状况（例如癌症）中起着重要作用。主要的ROS/RN，即，过氧化氢，过氧亚硝酸盐离子，一氧化氮和亚硝酸盐可以在不同的电极电位上氧化，因此可以通过电分析技术检测和定量。纳米尺寸的电化学探针特别适合测量单细胞和细胞细胞器中的ROS/RN。在本文中，我们调查了单个细胞内ROS/RN的局部测量结果的最新进展，并讨论了几个方法论问题，包括优化纳米电极几何形状，精确定位电池内部电化学探针以及对电分析数据的解释。
Reactive oxygen and nitrogen species (ROS and RNS) play important roles in various physiological processes (e.g., phagocytosis) and pathological conditions (e.g., cancer). The primary ROS/RNS, viz., hydrogen peroxide, peroxynitrite ion, nitric oxide, and nitrite ion, can be oxidized at different electrode potentials and therefore detected and quantified by electroanalytical techniques. Nanometer-sized electrochemical probes are especially suitable for measuring ROS/RNS in single cells and cellular organelles. In this article, we survey recent advances in localized measurements of ROS/RNS inside single cells and discuss several methodological issues, including optimization of nanoelectrode geometry, precise positioning of an electrochemical probe inside a cell, and interpretation of electroanalytical data.
