论文标题
SARS-COV-2的SARS-COV发病机理小病毒RNA的直系同源物
SARS-CoV-2 orthologs of pathogenesis-involved small viral RNAs of SARS-CoV
论文作者
论文摘要
背景：COVID-19的大流行时钟正在滴答作用，许多人类现代机构的生存和许多人的生存受到威胁。需要治疗可以显着降低Covid-19的发病率和死亡率。因此，我们深入研究了SARS-COV-2基因组，这是导致COVID-19的病毒。 SARS-COV-2来自与SARS-COV的同一家族，最近发现了三个小病毒RNA（SVRNA）。这些SVRNA在小鼠的病毒发病机理中起着重要作用。 贡献：在本文中，我们报告了SARS-COV-2基因组中这三个SVRNA的潜在直系同源物。我们使用的是直系同源物的现成搜索和对齐算法，而是使用了特殊的对齐评分，而不会对C/T和A/G不匹配的其他突变不像其他突变那样多。 RNA碱基C和U都可以与G结合；同样，A和G都可以与U结合，因此我们的评分。我们还使用新型独立的计算实验来验证这一假设。为了验证我们的结果，我们证实了发现的直系同源物在所有经过测试过的SARS-COV-2菌株的公开基因组中完全保存； SARS-COV-2直系同源物发生的基因座接近发生SARS-COV SVRNA的基因座。我们还报告了这些SVRNA的潜在目标。我们假设发现的直系同源物在SARS-COV-2的发病机理中起作用，因此，Antagomir介导的对这些SARS-COV-2 SVRNA的抑制作用抑制了Covid-19。
Background: The COVID-19 pandemic clock is ticking and the survival of many of mankind's modern institutions and or survival of many individuals is at stake. There is a need for treatments to significantly reduce the morbidity and mortality of COVID-19. Hence, we delved deep into the SARS-CoV-2 genome, which is the virus that has caused COVID-19. SARS-CoV-2 is from the same family as SARS-CoV in which three small viral RNAs (svRNA) were recently identified; those svRNAs play a significant role in the virus pathogenesis in mice. Contribution: In this paper, we report potential orthologs of those three svRNAs in the SARS-CoV-2 genome. Instead of off-the-shelf search and alignment algorithms, which failed to discover the orthologs, we used a special alignment scoring that does not penalize C/T and A/G mismatches as much as the other mutations. RNA bases C and U both can bind to G; similarly, A and G both can bind to U, hence, our scoring. We also validate this hypothesis using a novel, independent computational experiment. To validate our results, we confirmed the discovered orthologs are fully conserved in all the tested publicly available genomes of various strains of SARS-CoV-2; the loci at which the SARS-CoV-2 orthologs occur are close to the loci at which SARS-CoV svRNAs occur. We also report potential targets for these svRNAs. We hypothesize that the discovered orthologs play a role in pathogenesis of SARS-CoV-2, and therefore, antagomir-mediated inhibition of these SARS-CoV-2 svRNAs inhibits COVID-19.
