论文标题
NOD-SCID小鼠的4T1肿瘤的光动力疗法
Photodynamic therapy of 4T1 tumors in NOD-SCID mice
论文作者
论文摘要
背景：通过光动力疗法（PDT）治疗乳腺癌（PDT）的有希望的结果，前提是仔细选择辐射光波长。方法：植入在NOD-SCID小鼠中的4T1肿瘤用MetVix-PDT在625 nm，660 nm及其组合光中处理，以进行固定的辐射暴露。通过基于蒙特卡洛的计算模拟以及进行初步的体内研究来评估治疗结果，其中进行了荧光，大小和温度测量。结果：光源组合方案具有巨大的潜力，因为它导致高细胞毒性产品水平和减少的治疗时间；关于收获的肿瘤质量的体内发现也支持这一假设。单独使用625 nm束的辐照为大多数体内测量参数提供了更好的结果。仅用660 nm光源处理的小鼠具有最高的未透明光敏光敏剂（PS）信号，体温最低，最重的收获肿瘤和PDT细胞毒性产物的估计浓度最低。结论：使用625 nm辐照光与PS激发带匹配，但仅用于治疗表面肿瘤。对于更深的铺设质量，由于其渗透深度的增加，同时使用较长的波长会增强治疗结果。
Background: Promising results for mammary carcinoma treatment with photodynamic therapy (PDT) presuppose a careful selection of irradiation light wavelength. Methods: 4T1 tumors implanted in NOD-SCID mice were treated with Metvix-PDT under 625 nm, 660 nm and their combination light, for a fixed radiant exposure. The therapeutic outcome was assessed through Monte Carlo based computational simulations along with a preliminary in vivo study, where fluorescence, size and temperature measurements were conducted. Results: The light source combination protocol presents great potential, since it results in high cytotoxic products levels and reduced treatment times; while the in vivo findings, regarding the harvested tumor mass, also support this hypothesis. The irradiation with 625 nm beam alone presented better results for most of the in vivo measured parameters. The mouse treated with only the 660 nm light source had the highest un-photobleached photosensitizer (PS) signal, the lowest body temperature, the heaviest harvested tumor and the lowest estimated concentration of PDT cytotoxic products. Conclusions: The use of 625 nm irradiation light matches the PS excitation band but is preferable only for treatment of superficial tumors. For deeper laying masses, the simultaneous use of longer wavelengths enhances the therapeutic outcome due to their increased penetration depth.
