论文标题
在计算共聚焦显微镜中进行焦点优化
Focus optimization in a Computational Confocal Microscope
论文作者
论文摘要
我们考虑了共聚焦显微镜的计算扩展的性能的数值优化。使用针孔检测器替换探测器阵列的系统，我们试图为扫描中的每个点开发此其他信息。我们得出了对焦点的最佳估计值，从而最大程度地降低了异常光的贡献。我们估计在理论上可以在点扫描和扫描系统中进行的改进量，并通过仿真进行演示。我们发现，即使有很大程度的正则化，也可以进行重大改进，尤其是对于线扫描系统。
We consider the numerical optimization of performance for a computational extension of a confocal microscope. Using a system where the pinhole detector is replaced with a detector array, we seek to exploit this additional information for each point in the scan. We derive an optimal estimate of the light at focus which minimizes the contribution of out-of-focus light. We estimate the amount of improvement that would be theoretically possible in point-scanning and line-scanning systems and demonstrate with simulation. We find that even with a large degree of regularization, a significant improvement is possible, especially for line-scanning systems.
