论文标题

通过光学传输函数重塑3D对象的定量阶段和折射率成像

Quantitative phase and refractive index imaging of 3D objects via optical transfer function reshaping

论文作者

Hugonnet, Herve, Lee, Mahn Jae, Park, Yongkeun

论文摘要

反卷积阶段显微镜通过重建其传输相或折射率来对透明样品进行高对比度可视化。在此,我们提出了一种将2D反卷积相显微镜扩展到厚3D样品的方法。可以通过仅测量在优化的照明模式下获得的四个强度图像来在特定轴向平面上获得样品的折射率分布。同样,可以使用不同的照明模式来测量样品的光相延迟。

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to thick 3D samples. The refractive index distribution of a sample can be obtained at a specific axial plane by measuring only four intensity images obtained under optimized illumination patterns. Also, the optical phase delay of a sample can be measured using different illumination patterns.

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