论文标题
通过多模纤维的多视散射扫描成像共聚焦显微镜
Multiview Scattering Scanning Imaging Confocal Microscopy through a Multimode Fiber
论文作者
论文摘要
共聚焦和多光子显微镜是获得散装组织中2-D切片的高对比度图像的有效技术。但是，散射将其应用限制在最高约1毫米的深度。多模纤维产生了出色的超薄内窥镜，可以以最小的损害在组织内部深处穿透。在这里，我们提出了多视散射扫描成像共焦（MUSSIC）显微镜，该显微镜通过多模纤维实现高信噪比（SNR）成像，因此将光学切片和分辨率的分辨率与共斑显微镜的分辨率与多模纤维的最小侵入性渗透能力相结合。此处介绍的关键进步是通过使用多个共面虚拟针孔来捕获对象的多个观点，将其重新移动并合并以获得高对比度和高分辨率共焦图像来捕获对象的多个观点，从而实现了高SNR图像重建。我们介绍了在脑部显微镜中的对比和分辨率上获得增益的理论，并通过实验结果验证了概念。
Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent ultrathin endoscopes that can penetrate deep inside the tissue with minimal damage. Here, we present Multiview Scattering Scanning Imaging Confocal (MUSSIC) Microscopy that enables high signal-to-noise ratio (SNR) imaging through a multimode fiber, hence combining the optical sectioning and resolution gain of confocal microscopy with the minimally invasive penetration capability of multimode fibers. The key advance presented here is the high SNR image reconstruction enabled by employing multiple coplanar virtual pinholes to capture multiple perspectives of the object, re-shifting them appropriately and combining them to obtain a high-contrast and high-resolution confocal image. We present the theory for the gain in contrast and resolution in MUSSIC microscopy and validate the concept through experimental results.
