学术文献库

Lipid bilayer membranes are the fundamental biological barriers that permit life. The bilayer dynamics largely participates in orchestrating cellular workings and is characterized by substantial stability together with extreme plasticity that allows controlled morphological/topological changes. Modeling and understanding the topological change of vesicle-like membrane at the scale of a full cell has proved an elusive aim. We propose and discuss here a continuum model able to encompass the fusion/fission transition of a bilayer membrane at the scale of a Large Unilamellar Vesicle and evaluate the minimal free energy path across the transition, inspired by the idea that fusion/fission-inducing proteins have evolved in Nature towards minimal work expenditure. The results predict the correct height for the energetic barrier and provide the force field that, by acting on the membrane, can induce the transition. They are found in excellent agreement, in terms of intensity, scale, and spatial localization with experimental data on typical protein systems at play during the transition. The model may pave the way for the development of more complete models of the process where the dynamics is coupled to the fluid internal and external environment and to other applications where the topological changes do either occur or should be prevented.