论文标题
通道几何形状和流速在流体动力学上的影响，重点是循环肿瘤细胞的阻抗检测
Effect of Channel Geometry and Flow Rates in Hydrodynamic Focusing on Impedance Detection of Circulating Tumor Cells
论文作者
论文摘要
除生物学特性以外的细胞具有不同的电和物理特性。在阻抗细胞仪中，细胞应在位于电极对的检测区域中一个一个一个。当细胞位于电极之间时，阻抗会发生变化，这可以指示细胞的存在。这基本上是因为细胞的电特性与电极之间的介质不同，这对于确定阻抗很重要。影响阻抗细胞仪性能性能的最重要方面之一是微通道设计。在这项工作的第一步中，微通道的设计方式是在阻抗细胞仪中具有最佳检测的方式。在这方面，选择了流体动力焦点以聚焦从主通道入口进入的细胞群体。为了找到微通道的最佳参数，模拟了通道本身的不同几何形状，以及与护套流有关的流速和其他参数。在下一步中，在Comsol中测量了白细胞，MCF7和MDA-MB-231乳腺癌细胞的阻抗。结果表明，通过使用优化的通道设计测量细胞的阻抗，CTC可以成功区分WBC。
Cells, other than their biological properties, have different electric and physical properties. In an impedance cytometer, cells should pass one by one in the detection region where pairs of electrodes are located. When cells are located between electrodes, the impedance changes, and this can be indicative of the presence of a cell. This is basically because the electric properties of cells are different from the medium between the electrodes which is important in determining the impedance. One of the most important aspects which influence the performance of an impedance cytometer performance is the microchannel design. In this work, in the first step, the microchannel was designed in a way to have the best detection in the impedance cytometer. In this regard, hydrodynamic focusing was selected to focus the population of cells entering from the inlet of the main channel. To find the optimal parameters of the microchannel, different geometry for the channel itself, along with flow rates and other parameters related to sheath flow were simulated. In the next step, impedance was measured in COMSOL for White blood cells, MCF7, and MDA-MB-231 breast cancer cells. The results show that by measuring the impedance of cells using the optimized channel design, CTCs can be successfully differentiated from WBCs.
