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Network intrusion detection systems (NIDS) are an essential defense for computer networks and the hosts within them. Machine learning (ML) nowadays predominantly serves as the basis for NIDS decision making, where models are tuned to reduce false alarms, increase detection rates, and detect known and unknown attacks. At the same time, ML models have been found to be vulnerable to adversarial examples that undermine the downstream task. In this work, we ask the practical question of whether real-world ML-based NIDS can be circumvented by crafted adversarial flows, and if so, how can they be created. We develop the generative adversarial network (GAN)-based attack algorithm NIDSGAN and evaluate its effectiveness against realistic ML-based NIDS. Two main challenges arise for generating adversarial network traffic flows: (1) the network features must obey the constraints of the domain (i.e., represent realistic network behavior), and (2) the adversary must learn the decision behavior of the target NIDS without knowing its model internals (e.g., architecture and meta-parameters) and training data. Despite these challenges, the NIDSGAN algorithm generates highly realistic adversarial traffic flows that evade ML-based NIDS. We evaluate our attack algorithm against two state-of-the-art DNN-based NIDS in whitebox, blackbox, and restricted-blackbox threat models and achieve success rates which are on average 99%, 85%, and 70%, respectively. We also show that our attack algorithm can evade NIDS based on classical ML models including logistic regression, SVM, decision trees and KNNs, with a success rate of 70% on average. Our results demonstrate that deploying ML-based NIDS without careful defensive strategies against adversarial flows may (and arguably likely will) lead to future compromises.