论文标题
部分可观测时空混沌系统的无模型预测
Measuring vesicle loading with holographic microscopy and bulk light scattering
论文作者
论文摘要
我们报告使用在线数字全息显微镜量化细胞大小脂肪囊泡的努力。此方法不需要荧光记者，荧光示踪剂或放射性示踪剂。单色LED光源代替常规照明来产生全息图，而不是明亮的田间图像。通过使用Lorenz-Mie光散射模型对囊泡的散射进行建模，并将结果与数据全息图进行比较，我们能够测量囊泡的屈光度指数并因此加载。对散装光散射测量进行相同的比较，可以检索纳米级囊泡的囊泡载荷。
We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modelling the vesicle's scattering in a microscope with a Lorenz-Mie light scattering model, and comparing the results to data holograms, we are able to measure the vesicle's refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables retrieval of vesicle loading for nanoscale vesicles.
