论文标题
DNA存储错误模拟器：用于模拟合成，存储，PCR和测序中错误的工具
DNA Storage Error Simulator: A Tool for Simulating Errors in Synthesis, Storage, PCR and Sequencing
论文作者
论文摘要
DNA具有许多有价值的特征，使其适用于长期存储介质，尤其是其耐用性和高信息密度。 DNA可以安全地存储数百年，几乎没有降解，而硬盘驱动器通常持续约5年。此外，如果将DNA储存的持续时间延长至可能将其延长到数千年中，则如果将其排入储存和冷却。 DNA技术的进步使得将整个Wikipedia存储在试管中并使用手持测序设备阅读该信息，尽管需要减少书面形式（合成）和阅读（测序）的缺陷，以使其可行作为主流存储介质。新的测序技术，例如纳米孔测序，旨在渗透消费者世界，这要归功于它们的负担能力和规模。但是，纳米孔测序的误差特性尚未得到很好的表征。 DNA存储误差模拟器模型误差可以在DNA存储工作流的所有阶段中引入，包括合成，存储，用于扩增的PCR和最后测序。可以在所有必要的详细信息中配置测序和合成的误差特性，也可以根据可用文献和我们自己的分析从预定义值中选择。 可用性：DNA存储错误模拟器可以在线访问：https：//master.dbahb2jho41s4.amplifyapp.com（https：//dnastorage.ic.ic.ic.ic.ac.ac.ac.ac.ac.ac.ac.uk/dna-error-simimulator）
DNA has many valuable characteristics that make it suitable for a long-term storage medium, in particular its durability and high information density. DNA can be stored safely for hundreds of years with virtually no degradation, in contrast to hard disk drives which typically last for about 5 years. Furthermore, the duration of DNA-Storage can be extended to potentially up to thousands of years if it is desiccated and cooled in storage. Advances in DNA technologies have made it possible to store the entirety of Wikipedia in a test tube and read that information using a handheld sequencing device, although imperfections in writing (synthesis) and reading (sequencing) need to be mitigated for it to be viable as a mainstream storage medium. New sequencing technologies, such as nanopore sequencing, aim to penetrate the consumer world, thanks to their affordability and size. However, the error characteristics of nanopore sequencing are not yet well characterised. DNA Storage Error Simulator models errors that can be introduced in all the phases of DNA storage workflow, including synthesis, storage, PCR for amplification and finally sequencing. The error characteristics for sequencing and synthesis can be configured in all necessary detail or can be chosen from a predefined set of values based on available literature and our own analysis. Availability: DNA Storage Error Simulator can be accessed online from: https://master.dbahb2jho41s4.amplifyapp.com (https://dnastorage.doc.ic.ac.uk/DNA-error-simulator)
