论文标题

相位分离和动态阻止蛋白质溶液以短距离景点为主

Phase separation and dynamical arrest of protein solutions dominated by short-range attractions

论文作者

Hansen, Jan, Moll, Carolyn J., Flores, Leticia Lopez, Castaneda-Priego, Ramon, Medina-Noyola, Magdaleno, Egelhaaf, Stefan U., Platten, Florian

论文摘要

相位分离和动态停滞的相互作用会导致凝胶和眼镜的形成,这与诸如硬化和软凝结物理学,材料科学,食品工程和制药行业等多样化领域有关。在这里,分析了非平衡状态以及球状蛋白的相互作用。盐水中的溶菌酶被选为具有短距离吸引力的模型系统。通过云点测量,光学显微镜,离心实验和光散射,已经确定了各种溶液条件的亚稳态气液和动态停滞线以及第二个病毒系数$ B_2 $。如果以$ b_2 $表示温度,则在各种条件下获得的二线词落在主曲线上,如相应状态的扩展定律所建议。不同盐浓度的逮捕线在实验错误内重叠,而如果温度轴被$ b_2 $取代,则它们不会重叠。这表明二键对潜力的细节不敏感,但可以用一个积分参数来描述,即$ b_2 $,而逮捕线似乎受其有吸引力的部分支配。实验发现得到了非平衡自洽的广义Langevin方程理论的数值结果的支持。

The interplay of phase separation and dynamical arrest can lead to the formation of gels and glasses, which is relevant for such diverse fields as hard and soft condensed matter physics, materials science, food engineering and pharmaceutical industry. Here, the non-equilibrium states as well as the interactions of globular proteins are analyzed. Lysozyme in brine is chosen as a model system with short-range attractions. The metastable gas-liquid binodal and the dynamical arrest line as well as the second virial coefficient $B_2$ have been determined for various solution conditions by cloud-point measurements, optical microscopy, centrifugation experiments and light scattering. If temperature is expressed in terms of $B_2$, the binodals obtained under various conditions fall onto a master curve, as suggested by the extended law of corresponding states. Arrest lines for different salt concentrations overlap within experimental errors, whereas they do not overlap if the temperature axis is replaced by $B_2$. This indicates that the binodals are not sensitive to the details of the potential, but can be described by one integral parameter, i.e. $B_2$, whereas the arrest line appears governed by its attractive part. The experimental findings are supported by numerical results of the non-equilibrium self-consistent generalized Langevin equation theory.

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