论文标题
5分钟的磁颗粒光谱（MPS）基于生物测定：朝着超快速，方便和大规模疾病诊断
5-Minute Magnetic Particle Spectroscopy (MPS)-based Bioassay: Towards Ultra-fast, Convenient, and Large-scale Disease Diagnosis
论文作者
论文摘要
在这项工作中，我们报告了基于5分钟的磁颗粒光谱（MPS）的生物测定策略。在我们的方法中，将表面功能化的磁性纳米颗粒（MNP）与37°C的目标分析物一起搅拌3分钟，然后在第5分钟进行MPS读数。我们已经证明了5分钟的超快速检测SARS-COV-2峰值蛋白的可行性，检测极限低于5 nm（0.2 pmole）。我们提出的5分钟生物测定策略可用于减少其他液相，体积生物传感器（例如核磁共振）（NMR），量子点（QD），荧光生物传感器等的测定时间。
In this work, we report a 5-minute magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface functionalized magnetic nanoparticles (MNPs) are incubated with target analytes at 37 °C with agitation for 3 minutes and the MPS reading is then taken at the 5th minute. We have proved the feasibility of 5-minute ultra-fast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmole). Our proposed 5-minute bioassay strategy may be applied to reduce assay time for other liquid phase, volumetric biosensors such as nuclear magnetic resonance (NMR), quantum dots (QD), fluorescent biosensors, etc.
