论文标题
直接，一种用于荧光生物样品的高速低噪声成像的低成本系统
DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent biological samples
论文作者
论文摘要
已经开发了针对高时空分辨率固定样品记录的应用程序的目标成像系统。它通过在快速序列中照亮感兴趣的区域，并将整个视野中的信号记录到单个光电探测器上，并且可以在现有显微镜上以低成本实现信号，而不会损害现有功能。该系统的特征是在用于记录来自ASAP-3表达神经元的单个动作电位之前，以速度，空间分辨率和组织渗透深度为特征。
A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the whole field of view onto a single photodetector, and can be implemented at low cost on an existing microscope without compromising existing functionality. The system is characterized in terms of speed, spatial resolution, and tissue penetration depth, before being used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.
