论文标题
广场干涉降解剂增强中红外光热显微镜的单个病毒指纹识别
Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy
论文作者
论文摘要
病毒的临床鉴定和基本研究取决于病毒蛋白或病毒核酸的检测。然而，由于粒度较小和低含量的化学含量（例如，在囊泡炎性炎病毒中，〜5000个蛋白质），基于扩增和基于抗原的方法无法提供单个病毒体的精确组成信息。在这里，我们报告了宽场干涉剂量测量值增强的中红外光热（宽MIP）显微镜，用于单个病毒的高通量指纹。通过鉴定特征吸收峰，宽MIP揭示了单个DNA疫苗病毒和RNA囊泡性气孔病毒病毒蛋白和核酸的含量。获得胸骨和尿嘧啶残基振动的不同核酸特征，以区分DNA和RNA病毒。宽MIP成像进一步揭示了DNA水痘带状疱疹病毒蛋白中富集的\ b {eta}成分。这些进步共同开辟了一条新的途径，用于对病毒载体的组成分析和阐明组装的病毒体中的蛋白质功能。
Clinical identification and fundamental study of viruses rely on the detection of viral proteins or viral nucleic acids. Yet, amplification-based and antigen-based methods are not able to provide precise compositional information of individual virions due to small particle size and low-abundance chemical contents (e.g., ~ 5000 proteins in a vesicular stomatitis virus). Here, we report a widefield interferometric defocus-enhanced mid-infrared photothermal (WIDE-MIP) microscope for high-throughput fingerprinting of single viruses. With the identification of feature absorption peaks, WIDE-MIP reveals the contents of viral proteins and nucleic acids in single DNA vaccinia viruses and RNA vesicular stomatitis viruses. Different nucleic acids signatures of thymine and uracil residue vibrations are obtained to differentiate DNA and RNA viruses. WIDE-MIP imaging further reveals an enriched \b{eta} sheet components in DNA varicella-zoster virus proteins. Together, these advances open a new avenue for compositional analysis of viral vectors and elucidating protein function in an assembled virion.
