ICS 13.020.01
CCS Z01
团体 标准
T/CSES 80—2023
淡水生物 DNA条形码构建技术规程
Technical regulations for construction of DNA barcodes of freshwater organisms
发布稿
2023 - 01 - 04发布 2023 - 01 - 04实施
中国环境科学学会 发布
全国团体标准信息平台
T/CSES 80 —2023
I 目次
前言 ................................ ................................ ................................ ................................ ................................ ...... II
引言 ................................ ................................ ................................ ................................ ................................ .... III
1 范围 ................................ ................................ ................................ ................................ ................................ 1
2 规范性引用文件 ................................ ................................ ................................ ................................ ............ 1
3 术语和定义 ................................ ................................ ................................ ................................ .................... 1
4 试剂 ................................ ................................ ................................ ................................ ................................ 3
5 设备和材料 ................................ ................................ ................................ ................................ .................... 3
样品采集及前处理设备 ................................ ................................ ................................ ......................... 3
实验室分析设备 ................................ ................................ ................................ ................................ ..... 3
分析软件 ................................ ................................ ................................ ................................ ................. 3
其他辅助设备和材料 ................................ ................................ ................................ ............................. 3
6 DNA 条形码构建方法 ................................ ................................ ................................ ................................ .. 3
DNA条形码构建流程 ................................ ................................ ................................ ........................... 4
物种采集与鉴定 ................................ ................................ ................................ ................................ ..... 4
条形码扩增与测序 ................................ ................................ ................................ ................................ . 4
条形码评估 ................................ ................................ ................................ ................................ ............. 5
物种条形码 ................................ ................................ ................................ ................................ ............. 5
7 质量控制与质量保证 ................................ ................................ ................................ ................................ .... 5
质量控制 ................................ ................................ ................................ ................................ ................. 5
质量保证 ................................ ................................ ................................ ................................ ................. 5
8 废弃物处理 ................................ ................................ ................................ ................................ .................... 6
附录 A(资料性)常用 DNA条形码引物及 PCR扩增条件 ................................ ................................ .......... 7
附录 B(规范性)遗传距离分析原理和方法 ................................ ................................ ................................ .. 9
附录 C(资料性) DNA条形码数据表格 ................................ ................................ ................................ ....... 12
全国团
T-CSES 80—2023 淡水生物DNA条形码构建技术规程
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