TECHNICAL ISO/TS SPECIFICATION 6579-2 First edition 2012-11-01 Microbiology of food and animal feed Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 2: Enumeration by a miniaturized most probable number technique Microbiologie des aliments - Methode horizontale pour la recherche, le dénombrement et le serotypage des Salmonella- Partie 2: Denombrement par une technique miniaturisee du nombre le plus probable Reference number ISO/TS 6579-2:2012(E) ISO @ ISO 2012 ISO/TS 6579-2:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO 2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland = @ ISO 2012 -All rights reserved ISO/TS6579-2:2012(E) Contents Page Foreword ..iv Introduction 1 Scope 2 Normative references 3 Terms and definitions 2 4 Principle 2 4.1 General 2 4.2 Pre-enrichment in non-selective liquid medium 4.3 Enrichment on a selective semi-solid medium 4.4 Selective plating and identification .2 4.5 Confirmation 4.6 Calculation of most probable number 5 Culture media and sera. 3 5.1 General 5.2 Culture media. 6 Apparatus and glassware 3 7 Sampling.. 8 Preparation of test sample. 9 Procedure 9.1 Test portion, initial suspension ... 9.2 Dilution and pre-enrichment in non-selective liquid medium 5 9.3 Selectiveenrichmentonasemi-solidmedium 9.4 Selective plating .. 9.5 Biochemical and serological confirmation. 6 10 Expression of results .8 11 Testreport 8 Annex A (informative) Composition and preparation of culture media and reagents 9 Annex B (informative) Diagram of procedure .17 Bibliography 18 @ ISO 2012 -All rights reserved ili