International ISO Standard IS07836 First edition NaturalrubberIdentification of 2024-07 phytosterols, including β-sitosterol Caoutchouc naturel-Identificationdesphytosterols, en particulierduβ-sitosterol Referencenumber IS07836:2024(en) @ISO2024 IS02024 or Iso's member body in the country of the requester. ISO copyright office CP 4oi : Ch.de Blandonnet 8 CH-1214 Vernier,Geneva Phone: +41 22 749 01 11 Email: copyright@iso.org Website: www.iso.org Published in Switzerland @ISo 2024-Allrightsreserved ii Contents Page Foreword. ..iv 1 Scope .1 2 Normativereferences .1 3 Terms and definitions. .1 4 Principle .1 5 Reagents. .2 6 Apparatus .2 7 Procedure .3 7.1 Samplepreparation .3 7.2 Thin layer chromatographytechnique .3 7.2.1 Preparation of the TLC plates. .3 7.2.2 Marking the TLC plates. .3 3 7.2.3 Spottingthe TLC plates 7.2.4 Developing theTLC plates. .3 7.2.5 Identificationofβ-sitosterol. .3 8 Expression of results. .4 9 Test report. .4 @IS0 2024-All rights reserved iii